Haematology Laboratory Services

{tab=Routine Haematology}

Routine Haematology Section

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
FBC (Full Blood Count)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	1 Hour 30 min Turn around time
DC (Differential Count)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
FBP (Full Blood Picture)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Refer request indication Office Hour ONLY, before 4.30pm
BT (Bleeding Time)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Make appointment at the lab Office Hour ONLY, before 4.30pm

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

FBC

(Full Blood Count)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

1 Hour 30 min

Turn around time

(Differential Count)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

FBP

(Full Blood Picture)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

Refer request indication

Office Hour ONLY, before 4.30pm

(Bleeding Time)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

Make appointment at the lab

Office Hour ONLY, before 4.30pm

Note; FBP request made after Office Hour must to be informed to the lab by call before sending the request, except for neonate sample

{tab=Routine Coagulation}

Haemostasis Section

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
PT (Prothrombin Time)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
INR (International Normalized Ratio)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
APTT (Activated Partial Thromboplastin Time)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
D-DIMER	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
DIC SCREENING: PT/INR/APTT Fibrinogen Level D-dimer Fibrin monomer	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
TT (Thrombine Time)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

(Prothrombin Time)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

INR

(International Normalized Ratio)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

APTT

(Activated Partial Thromboplastin Time)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

D-DIMER

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

DIC SCREENING:

PT/INR/APTT
Fibrinogen Level
D-dimer
Fibrin monomer

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

(Thrombine Time)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within 4 hours of blood collection

{/tabs}

{tab=Specialised Haematology}

{slide=Haemolysis}

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
G6PD	Filter paper dried blood	FIlter paper	Fully dry blood on filter paper and place it in biohazard plastic bag
OFT	Blood	Heparin tube	Make appointment
KLEIHAUER TEST	Mother's blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Make appointment

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

G6PD

Filter paper dried blood

FIlter paper

Fully dry blood on filter paper and place it in biohazard plastic bag

OFT

Blood

Heparin tube

Make appointment

KLEIHAUER TEST

Mother's blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

Make appointment

{/slide}

{slide=Haemoglobin Analysis}

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
HB Electrophoresis	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
HB H Inclusion	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
SICKLING TEST	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
HPLC analysis (High Performance Liquid Chromatography)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-

{/slide}

{slide=Nutritional Anaemia}

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
SERUM FERRITIN	Blood	Plain tube (red cap) 3mL adult, 2mL child, 0.5mL baby	-
RBC FOLATE SERUM FOLATE VITAMIN B12	This test was not done since 22 Jan 2011 Request for this test must be to an outside lab	NA	-

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

SERUM FERRITIN

Blood

Plain tube (red cap)

3mL adult, 2mL child, 0.5mL baby

RBC FOLATE

SERUM FOLATE

VITAMIN B12

This test was not done since 22 Jan 2011

Request for this test must be to an outside lab

{/slide}

{tab=Specialised Coagulation}

Haemostasis Section

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
INHIBITOR ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	2 tubes
THROMBOPHILIA	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	4 tubes
Von WILLEBRAND	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	3 tubes
PLATELET AGGREGATION	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	4 tubes - whole blood 8 tubes - platelet rich plasma
ANTI XA ACTIVITY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	1 tube, refer lab for sample collection
MIXING TEST	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	2 tubes
FACTOR II ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR V ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR VII ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR VIII ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR IX ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR X ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR XI ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR XII ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR XIII SCREENING	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
LUPUS ANTICOAGULANT	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL

All specialised coagulation tests cannot use blood sample in 1.0mL 3.2% 0.109M Trisdium Citrate tubes.

All samples must reach lab before 4 hours of blood collection sent without ice

All specialised coagulation tests must be made via appointment, please call lab (ext: 4020)

{/tabs}

{tab=Bone Marrow}

{tab=Flowcytometry}

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
PNH (Paroxymal Nocturnal Hemoglobinuria)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Please make an appointment
Leukaemia Phenotyping	Blood / Bone Marrow	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Appointment is not required, Send the sample immediately
Lymphoma	Blood / Body Fluid / Tissue	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Please make an appointment Send the sample immediately Use sterile container for body fluid sample
Myeloma	Blood / Body Fluid / Tissue	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Please make an appointment Send the sample immediately Use sterile container for body fluid sample

{tab=Molecular}

TEST	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
BCR-ABL Qualitative Gene	Blood / Bone Marrow	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
JAK 2 Gene	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
AML Translocation Gene AML / ETO / PML / RARα	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
SEA α-Thalassemia	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
Factor V Leiden	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)

{/tabs}