SUMMARY GUIDELINE FOR HAEMATOLOGY TEST

TYPE OF TESTS	REQUEST TYPE	REQUEST HOUR	TYPE OF SPECIMEN	CONTAINER USE	VOLUME OF SPECIMEN	PREPARATION AND COLLECTION SPECIMEN
FULL BLOOD COUNT (FBC)	WITH LIS + Without request form	24 hours	Routine Test + STAT + Without Appointment	K2 EDTA	- 3ml Adult - 2ml Pediatric - 0.5ml Neonate	- Biohazard Plastic - WITHOUT ICE
DIFFERENTIAL COUNT (DC)
FULL BLOOD PICTURE (FBP)
ERYTHROCYTE SEDIMENTATION RATE (ESR)					3ml Adult
BLEEDING TIME (BT)	WITH LIS + With request form	Office Hour Only	Special Test + With Appointment	Ear Prick (Duke’s Method)	Ear Prick (Duke’s Method)	-
- PT /INR - APTT - D-DIMER - FIBRINOGEN LEVEL	WITH LIS + Without request form	24 hours	Routine Test + STAT + Without Appointment	3.2%, 0.109M Trisodium Citrate	- 2.7ml Adult - 1.8ml Pediatric - 1.0ml Neonate	- Biohazard Plastic - With Ice if the collection time from ward/clinic more than 30minutes to sent to the lab and/or the temperature exceed room temperature (19®C- 25®C)
DIC SCREENING - PT /INR - APTT - D-DIMER - FIBRINOGEN LEVEL - FIBRIN MONOMER	WITH LIS + Without request form	24 hours	Routine Test + STAT + Without Appointment		- 2.7ml Adult - 1.8ml Pediatric - 1.0ml Neonate
FACTOR II ASSAY	WITH LIS + With request form	Office Hour Only	Special Test + With Appointment		- 2.7ml Adult (1Tube) - 1.8ml Pediatric/Neonate (1 Tube)
FACTOR V ASSAY
FACTOR VII ASSAY
FACTOR VIII ASSAY
FACTOR IX ASSAY
FACTOR X ASSAY
FACTOR XI ASSAY
FACTOR XII ASSAY
FACTOR XIII SCREENING
LUPUS ANTICOAGULANT				3.2%, 0.109M Trisodium Citrate	- 2.7ml Adult (2 Tube) - 1.8ml Pediatric/Neonate (3 Tube)
MIXING TEST					2.7ml Adult/ Pediatric (2 Tube)
INHIBITOR ASSAY					2.7ml Adult/ Pediatric (2 Tube)
THROMBOPHILIA					2.7ml Adult/Pediatric (4 Tube)
VON WILLEBRAND	WITH LIS + With request form		Special Test + With Appointment		2.7ml Adult/Pediatric (3 Tube)
PLATELET AGGREGATION		Office Hour Only			2.7ml Adult/Pediatric - 8 Tube – PRP - 4 Tube – Whole Blood	- Biohazard Plastic - WITHOUT ICE - SENT IMMEDIATELY
ANTI XA ACTIVITY		Office Hour Only			2.7ml Adult/Pediatric (1 Tube)
GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PD)	WITH LIS + Without request form	24 hours	Routine Test + STAT + With Appointment	Dried blood	Filter paper	- Biohazard Plastic - WITHOUT ICE
OSMOTIC FRAGILITY TEST (OFT)	WITH LIS + With request form	Office Hour Only	Special Test + With Appointment	Heparin	3ml Adult
KLEIHAUER TEST	WITH LIS + With request form		Special Test + With Appointment	K2 EDTA (mother)	3ml Adult
HB ELECTROPHORESIS (HB ANALYSIS)	WITH LIS + Without request form		Special Test + Without Appointment	K2 EDTA	- 3ml Adult - 2ml Pediatric - 0.5ml Neonate
HB H INCLUSIONS	WITH LIS + With request form		Special Test + Without Appointment
SICKLING TEST	WITH LIS + With request form		Special Test + Without Appointment
HPLC	WITH LIS + Without request form		Special Test + Without Appointment	K2 EDTA	- 3ml Adult - 2ml Pediatric - 0.5ml Neonate	- Biohazard Plastic - WITHOUT ICE
ASPIRAT SUM-SUM TULANG (MGG)	WITH LIS + With request form		Special Test + With Appointment	Bone Marrow	Direct Smear
PERIODIC ACID SCHIFF REACTION			Special Test + With Appointment	- Bone Marrow /Blood - K2 EDTA	- 3ml Adult - 2ml Pediatric - 0.5ml Neonate
PEROXIDASE STAIN			Special Test + With Appointment	- Bone Marrow /Blood - K2 EDTA	- 3ml Adult - 2ml Pediatric - 0.5ml Neonate	- Biohazard Plastic - WITHOUT ICE
ACID PHOSPHATASE			Special Test + With Appointment
DUAL ESTERASE			Special Test + With Appointment
NEUTROPHIL ALKALINE PHOSPHATE SCORE			Special Test + With Appointment	- Blood - K2 EDTA
SERUM FERRITIN	WITH LIS + Without request form		Special Test + Without Appointment	Plain Tube (Red Stopper)	- 3ml Adult - 2ml Pediatric - 0.5ml Neonate
PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH)	WITH LIS + With request form		Special Test + With Appointment	- Blood - K2 EDTA	- 3ml Adult - 2ml Pediatric - 0.5ml Neonate (SENT IMMEDIATELY)
LEUKEMIA LYMPHOMA MYELOMA	WITH LIS + With request form			- Bone Marrow /Blood - K2 EDTA
LEUKEMIA LYMPHOMA MYELOMA	WITH LIS + With request form			- Body Fluid/Tisu - K2 EDTA		Sterile Container
GEN BCR-ABL QUALITATIVE	WITH LIS + With request form	Office Hour Only	Special Test + With Appointment	- Blood/Bone Marrow - K2 EDTA	3ml Adult (1 Tube)	- Biohazard Plastic - WITH ICE if specimen from outside hospital
GEN JAK2			Special Test + With Appointment	- Blood/Bone Marrow - K2 EDTA
GEN AML TRANSLOCATION (AML/ETO, PML/RARΑ/CBFΒ/MYH11)			Special Test + With Appointment	- Blood - K2 EDTA
ALPHA THALASSEMIA		Office Hour Only	Special Test + With Appointment	- Blood - K2 EDTA	3ml Adult (1 Tube)	- Biohazard Plastic - WITH ICE if specimen from outside hospital
FACTOR V LEIDEN		Office Hour Only	Special Test + Without Appointment	- Blood - K2 EDTA	3ml Adult (1 Tube)

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
FBC (Full Blood Count)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	1 Hour 30 min Turn around time
DC (Differential Count)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
FBP (Full Blood Picture)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Refer request indication Office Hour ONLY, before 4.30pm
BT (Bleeding Time)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Make appointment at the lab Office Hour ONLY, before 4.30pm

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

FBC

(Full Blood Count)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

1 Hour 30 min

Turn around time

(Differential Count)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

FBP

(Full Blood Picture)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

Refer request indication

Office Hour ONLY, before 4.30pm

(Bleeding Time)

Blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

Make appointment at the lab

Office Hour ONLY, before 4.30pm

Note; FBP request made after Office Hour must to be informed to the lab by call before sending the request, except for neonate sample

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
PT (Prothrombin Time)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
INR (International Normalized Ratio)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
APTT (Activated Partial Thromboplastin Time)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
D-DIMER	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
DIC SCREENING: PT/INR/APTT Fibrinogen Level D-dimer Fibrin monomer	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection
TT (Thrombine Time)	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.0mL baby	Send without ice within 4 hours of blood collection

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

(Prothrombin Time)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

INR

(International Normalized Ratio)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

APTT

(Activated Partial Thromboplastin Time)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

D-DIMER

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

DIC SCREENING:

PT/INR/APTT
Fibrinogen Level
D-dimer
Fibrin monomer

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within

4 hours of blood collection

(Thrombine Time)

Blood (plasma)

3.2% 0.109M Trisodium Citrate

2.7mL adult, 1.8mL child, 1.0mL baby

Send without ice within 4 hours of blood collection

HEMOLISIS

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
G6PD	Filter paper dried blood	FIlter paper	Fully dry blood on filter paper and place it in biohazard plastic bag
OFT	Blood	Heparin tube	Make appointment
KLEIHAUER TEST	Mother's blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Make appointment

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

G6PD

Filter paper dried blood

FIlter paper

Fully dry blood on filter paper and place it in biohazard plastic bag

OFT

Blood

Heparin tube

Make appointment

KLEIHAUER TEST

Mother's blood

K₂EDTA

3mL adult, 2mL child, 1.5mL baby

Make appointment

HAEMOGLOBIN ANALYSIS

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
HB Electrophoresis	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
HB H Inclusion	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
SICKLING TEST	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-
HPLC analysis (High Performance Liquid Chromatography)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	-

NUTRITIONAL ANAEMIA (THIS TEST IS OFFERD IN CDL, HOSPITAL USM)

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
SERUM FERRITIN	Blood	Plain tube (red cap) 3mL adult, 2mL child, 0.5mL baby	-
RBC FOLATE SERUM FOLATE VITAMIN B12	This test was not done since 22 Jan 2011 Request for this test must be to an outside lab	NA	-

TESTS

TYPE OF SPECIMEN

COLLECTION TUBE

NOTE

SERUM FERRITIN

Blood

Plain tube (red cap)

3mL adult, 2mL child, 0.5mL baby

RBC FOLATE

SERUM FOLATE

VITAMIN B12

This test was not done since 22 Jan 2011

Request for this test must be to an outside lab

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
INHIBITOR ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	2 tubes
THROMBOPHILIA	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	4 tubes
Von WILLEBRAND	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	3 tubes
PLATELET AGGREGATION	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	4 tubes - whole blood 8 tubes - platelet rich plasma
ANTI XA ACTIVITY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	1 tube, refer lab for sample collection
MIXING TEST	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL ONLY	2 tubes
FACTOR II ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR V ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR VII ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR VIII ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR IX ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR X ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR XI ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR XII ASSAY	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
FACTOR XIII SCREENING	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL
LUPUS ANTICOAGULANT	Blood (plasma)	3.2% 0.109M Trisodium Citrate 2.7mL adult, 1.8mL child, 1.8mL baby	2 tubes of 2.7mL 3 tubes of 1.8mL

All specialised coagulation tests cannot use blood sample in 1.0mL 3.2% 0.109M Trisdium Citrate tubes.

All samples must reach lab before 4 hours of blood collection sent without ice

All specialised coagulation tests must be made via appointment, please call lab (ext: 4020)

TBA

TESTS	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
PNH (Paroxymal Nocturnal Hemoglobinuria)	Blood	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Please make an appointment
Leukaemia Phenotyping	Blood / Bone Marrow	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Appointment is not required, Send the sample immediately
Lymphoma	Blood / Body Fluid / Tissue	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Please make an appointment Send the sample immediately Use sterile container for body fluid sample
Myeloma	Blood / Body Fluid / Tissue	K₂EDTA 3mL adult, 2mL child, 1.5mL baby	Please make an appointment Send the sample immediately Use sterile container for body fluid sample

TEST	TYPE OF SPECIMEN	COLLECTION TUBE	NOTE
BCR-ABL Qualitative Gene	Blood / Bone Marrow	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
JAK 2 Gene	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
AML Translocation Gene AML / ETO / PML / RARα	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
SEA α-Thalassemia	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)
Factor V Leiden	Blood	K2EDTA, 3mL only	without ice (request from HUSM patient) with ice (request from patient outside of HUSM)

TYPE OF TEST & SPECIMEN	TYPE & VOLUME	COLLECTION	REQUEST TYPE & HOUR
ROUTINE & STAT TESTS NO APPOINTMENT REQUIRED: 1. Full Blood Count (FBC) 2. Differential Count (DC) 3. Full Blood Picture (FBP) 4. Erythrocyte Sedimentation Rate (ESR)	K2 EDTA: - 3mL, adult - 2mL, pead - 0.5mL, neonate	- Biohazard plastic <br> - WITHOUT ICE	via LIS, no request form 24 hours
SPECIAL TESTS APPOINTMENT REQUIRED: Bleeding Time (BT)	Ear prick (Duke's Method)	-	via LIS, with request form office hours ONLY
ROUTINE & STAT TESTS NO APPOINTMENT REQUIRED: 1. PT / INR 2. APTT 3. D-Dimer 4. Fibrinogen Level	3.2% Trisodium Citrate Tube: - 2.7mL, adult - 1.8mL, pead - 1.0mL, neonate	- Biohazard plastic <br> - WITHOUT ICE	via LIS, no request form 24 hours
SPECIAL TESTS APPOINTMENT REQUIRED: 1. Factor II assay 2. Factor V assay 3. Factor VII assay 4. Factor VIII assay 5. Factor IX assay 6. Factor X assay 7. Factor XI assay 8. Factor XII assay 9. Factor XIII screening	3.2% Trisodium Citrate Tube: - 2.7mL, adult (1 tube) - 1.8mL, pead/neonate (1 tube)	- Biohazard plastic <br> - WITHOUT ICE	via LIS, no request form Office hours ONLY
SPECIAL TESTS APPOINTMENT REQUIRED: 1. Lupus anticoagulant 2. Mixing test 3. Inhibitor assay 4. Thrombophilia 5. von Willebrand 6. Factor X assay 7. Platelet Aggregation 8. Anti Xa activity	3.2% Trisodium Citrate Tube: - 2.7mL, adult (2 tube) - 1.8mL, pead/neonate (3 tube)	- Biohazard plastic <br> - WITHOUT ICE	via LIS, no request form Office hours ONLY