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Department of Hematology & Transfusion Medicine Unit

SUMMARY GUIDELINE FOR HAEMATOLOGY TEST

  • TYPE OF TESTS

    REQUEST TYPE

    REQUEST HOUR

    TYPE OF SPECIMEN

    CONTAINER USE

    VOLUME OF SPECIMEN

    PREPARATION AND COLLECTION SPECIMEN

    FULL BLOOD COUNT (FBC)

    WITH LIS + Without request form

    24 hours

    Routine Test + STAT + Without Appointment

    K2 EDTA

    -   3ml Adult

    -   2ml Pediatric

    -   0.5ml Neonate

    -   Biohazard Plastic

    -   WITHOUT ICE

    DIFFERENTIAL COUNT (DC)

    FULL BLOOD PICTURE (FBP)

    ERYTHROCYTE SEDIMENTATION RATE (ESR)

    3ml Adult

    BLEEDING TIME (BT)

    WITH LIS + With request form

    Office Hour Only

    Special Test + With Appointment

    Ear Prick (Duke’s Method)

    Ear Prick (Duke’s Method)

    -

    -  PT /INR

    -  APTT

    -  D-DIMER

    -  FIBRINOGEN LEVEL

    WITH LIS + Without request form

    24 hours

    Routine Test + STAT + Without Appointment

    3.2%, 0.109M Trisodium Citrate

    -   2.7ml Adult

    -   1.8ml Pediatric

    -   1.0ml Neonate

    -    Biohazard Plastic

    -    With Ice if the collection time from ward/clinic more than 30minutes to sent to the lab and/or the temperature exceed room temperature  (19®C- 25®C)

    DIC SCREENING

    -   PT /INR

    -   APTT

    -   D-DIMER

    -   FIBRINOGEN LEVEL

    -   FIBRIN MONOMER

    WITH LIS + Without request form

    Routine Test + STAT + Without Appointment

    FACTOR II ASSAY

    WITH LIS + With request form

    Office Hour Only

    Special Test + With Appointment

    -   2.7ml Adult (1Tube)

    -   1.8ml Pediatric/Neonate (1 Tube)

    FACTOR V ASSAY

    FACTOR VII ASSAY

    FACTOR VIII ASSAY

    FACTOR IX ASSAY

    FACTOR X ASSAY

    FACTOR XI ASSAY

    FACTOR XII ASSAY

    FACTOR XIII SCREENING

    LUPUS ANTICOAGULANT

    3.2%, 0.109M Trisodium Citrate

    -   2.7ml Adult (2 Tube)

    -   1.8ml Pediatric/Neonate

    (3 Tube)

    MIXING TEST

    2.7ml Adult/ Pediatric

    (2 Tube)

    INHIBITOR ASSAY

    THROMBOPHILIA

    2.7ml Adult/Pediatric

    (4 Tube)

    VON WILLEBRAND

    WITH LIS + With request form

    Special Test + With Appointment

    2.7ml Adult/Pediatric

    (3 Tube)

    PLATELET AGGREGATION

    Office Hour Only

    2.7ml Adult/Pediatric

    -     8 Tube – PRP

    -     4 Tube – Whole Blood

    -   Biohazard Plastic

    -   WITHOUT ICE

    -   SENT IMMEDIATELY

    ANTI XA ACTIVITY

    2.7ml Adult/Pediatric

    (1 Tube)

    GLUCOSE-6-PHOSPHATE

    DEHYDROGENASE (G6PD)

    WITH LIS + Without request form

    24 hours

    Routine Test + STAT + With Appointment

    Dried blood

    Filter paper

    -     Biohazard Plastic

    -     WITHOUT ICE

    OSMOTIC FRAGILITY TEST (OFT)

    WITH LIS + With request form

    Office Hour Only

    Special Test + With Appointment

    Heparin

    3ml Adult

    KLEIHAUER TEST

    Special Test + With Appointment

    K2 EDTA (mother)

    3ml Adult

    HB ELECTROPHORESIS (HB ANALYSIS)

    WITH LIS + Without request form

    Special Test + Without Appointment

    K2 EDTA

    -   3ml Adult

    -   2ml Pediatric

    -    0.5ml Neonate

    HB H INCLUSIONS

    WITH LIS + With request form

    Special Test + Without Appointment

    SICKLING TEST

    Special Test + Without Appointment

    HPLC

    WITH LIS + Without request form

    Special Test + Without Appointment

    K2 EDTA

    -   3ml Adult

    -   2ml Pediatric

    -     0.5ml Neonate

    -     Biohazard Plastic

    -     WITHOUT ICE

    ASPIRAT SUM-SUM TULANG (MGG)

    WITH LIS + With request form

    Special Test + With Appointment

    Bone Marrow

    Direct Smear

    PERIODIC ACID SCHIFF REACTION

    Special Test + With Appointment

    -  Bone Marrow /Blood

    -  K2 EDTA

    -   3ml Adult

    -   2ml Pediatric

    -   0.5ml Neonate

    PEROXIDASE STAIN

    Special Test + With Appointment

    -   Bone Marrow /Blood

    -   K2 EDTA

    -   3ml Adult

    -   2ml Pediatric

    -   0.5ml Neonate

    -     Biohazard Plastic

    -     WITHOUT ICE

    ACID PHOSPHATASE

    Special Test + With Appointment

    DUAL ESTERASE

    Special Test + With Appointment

    NEUTROPHIL ALKALINE PHOSPHATE SCORE

    Special Test + With Appointment

    -   Blood

    -   K2 EDTA

    SERUM FERRITIN

    WITH LIS + Without request form

    Special Test + Without Appointment

    Plain Tube

    (Red Stopper)

    -   3ml Adult

    -   2ml Pediatric

    - 0.5ml Neonate

    PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH)

    WITH LIS + With request form

    Special Test + With Appointment

    -   Blood

    -   K2 EDTA

    -   3ml Adult

    -   2ml Pediatric

    - 0.5ml Neonate

    (SENT IMMEDIATELY)

    LEUKEMIA LYMPHOMA

    MYELOMA

    WITH LIS + With request form

    -   Bone Marrow /Blood

    -   K2 EDTA

    -   Body Fluid/Tisu

    -   K2 EDTA

    Sterile Container

    GEN BCR-ABL QUALITATIVE

    WITH LIS + With request form

    Office Hour Only

    Special Test + Without Appointment

    -   Blood/Bone Marrow

    -   K2 EDTA

    3ml Adult (1 Tube)

    -   Biohazard Plastic

    -   WITH ICE if specimen from outside hospital

    GEN JAK2

    Special Test + Without Appointment

    GEN AML TRANSLOCATION

    (AML/ETO, PML/RARΑ/CBFΒ/MYH11)

    Special Test + Without Appointment

    -   Blood

    -   K2 EDTA

    ALPHA THALASSEMIA

    Office Hour Only

    Special Test + Without Appointment

    -   Blood

    -   K2 EDTA

    3ml Adult (1 Tube)

    -   Biohazard Plastic

    -   WITH ICE if specimen from outside hospital

    FACTOR V LEIDEN

    Office Hour Only

    Special Test + Without Appointment

    -   Blood

    -   K2 EDTA

    3ml Adult (1 Tube)

  •  

    TESTS TYPE OF SPECIMEN COLLECTION TUBE NOTE

    FBC

    (Full Blood Count)

    		 Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    1 Hour 30 min

    Turn around time

    DC

    (Differential Count)

    		 Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    		 -

    FBP

    (Full Blood Picture)

    		 Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    Refer request indication

    Office Hour ONLY, before 4.30pm

    BT

    (Bleeding Time)

    		 Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    Make appointment at the lab

    Office Hour ONLY, before 4.30pm

    Note; FBP request made after Office Hour must to be informed to the lab by call before sending the request, except for neonate sample

  •  

    TESTS TYPE OF SPECIMEN COLLECTION TUBE NOTE

    PT

    (Prothrombin Time)

    		 Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.0mL baby              

    Send without ice within

    4 hours of blood collection

    INR

    (International Normalized Ratio)

    		 Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.0mL baby

    Send without ice within

    4 hours of blood collection

    APTT

    (Activated Partial Thromboplastin Time)

    		 Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.0mL baby

    Send without ice within

    4 hours of blood collection
    D-DIMER Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.0mL baby

    Send without ice within

    4 hours of blood collection

    DIC SCREENING:

    • PT/INR/APTT
    • Fibrinogen Level
    • D-dimer
    • Fibrin monomer
    		 Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.0mL baby

    Send without ice within

    4 hours of blood collection

    TT

    (Thrombine Time)

    		 Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.0mL baby

    Send without ice within 4 hours of blood collection

  •  HEMOLISIS

    TESTS TYPE OF SPECIMEN COLLECTION TUBE NOTE
    G6PD Filter paper dried blood FIlter paper Fully dry blood on filter paper and place it in biohazard plastic bag
    OFT Blood Heparin tube Make appointment
    KLEIHAUER TEST Mother's blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    		 Make appointment

    HAEMOGLOBIN ANALYSIS

    TESTS TYPE OF SPECIMEN COLLECTION TUBE NOTE
    HB Electrophoresis Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    		 -
    HB H Inclusion Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    		 -
    SICKLING TEST Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    		 -

    HPLC analysis

    (High Performance Liquid Chromatography)

    		 Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    		 -

    NUTRITIONAL ANAEMIA (THIS TEST IS OFFERD IN CDL, HOSPITAL USM)

    TESTS TYPE OF SPECIMEN COLLECTION TUBE NOTE
    SERUM FERRITIN Blood

    Plain tube (red cap)

    3mL adult, 2mL child, 0.5mL baby

    		 -

    RBC FOLATE

    SERUM FOLATE

    VITAMIN B12

    This test was not done since 22 Jan 2011

    Request for this test must be to an outside lab

    		 NA -

     

  •  

    TESTS TYPE OF SPECIMEN COLLECTION TUBE NOTE
    INHIBITOR ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL ONLY

    		 2 tubes
    THROMBOPHILIA Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL ONLY

    		 4 tubes
    Von WILLEBRAND Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL ONLY

    		 3 tubes
    PLATELET AGGREGATION Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL ONLY

    4 tubes - whole blood

    8 tubes - platelet rich plasma
    ANTI XA ACTIVITY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL ONLY

    		 1 tube, refer lab for sample collection
    MIXING TEST Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL ONLY

    		 2 tubes
    FACTOR II ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR V ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR VII ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR VIII ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR IX ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR X ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR XI ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR XII ASSAY Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    FACTOR XIII SCREENING Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL
    LUPUS ANTICOAGULANT Blood (plasma)

    3.2% 0.109M Trisodium Citrate

    2.7mL adult, 1.8mL child, 1.8mL baby

    2 tubes of 2.7mL

    3 tubes of 1.8mL

    All specialised coagulation tests cannot use blood sample in 1.0mL 3.2% 0.109M Trisdium Citrate tubes.

    All samples must reach lab before 4 hours of blood collection sent without ice

    All specialised coagulation tests must be made via appointment, please call lab (ext: 4020)

  • TBA

  •  

    TESTS TYPE OF SPECIMEN COLLECTION TUBE NOTE

    PNH

    (Paroxymal Nocturnal Hemoglobinuria) 

    		 Blood

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    		 Please make an appointment
    Leukaemia Phenotyping Blood / Bone Marrow

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    Appointment is not required,

    Send the sample immediately 
    Lymphoma

    Blood /

    Body Fluid /

    Tissue

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    Please make an appointment

    Send the sample immediately

    Use sterile container for body fluid sample
    Myeloma

    Blood /

    Body Fluid /

    Tissue

    K2EDTA

    3mL adult, 2mL child, 1.5mL baby

    Please make an appointment

    Send the sample immediately

    Use sterile container for body fluid sample

     

  •  

    TEST TYPE OF SPECIMEN COLLECTION TUBE NOTE

    BCR-ABL

    Qualitative Gene

    		 Blood / Bone Marrow K2EDTA, 3mL only

    without ice (request from HUSM patient)

    with ice (request from patient outside of HUSM)
    JAK 2 Gene Blood K2EDTA, 3mL only

    without ice (request from HUSM patient)

    with ice (request from patient outside of HUSM)

    AML Translocation Gene

    AML / ETO / PML / RARα

    		 Blood K2EDTA, 3mL only

    without ice (request from HUSM patient)

    with ice (request from patient outside of HUSM)
    SEA α-Thalassemia Blood K2EDTA, 3mL only

    without ice (request from HUSM patient)

    with ice (request from patient outside of HUSM)
    Factor V Leiden Blood K2EDTA, 3mL only

    without ice (request from HUSM patient)

    with ice (request from patient outside of HUSM)

     

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